The artist has given the full amount of his £25k award prize money to the MVT, which functions to save the UK's small live music spots.
Fender was presented with the cheque on October 16 as his new release his work was named the outstanding release of the past year.
Fender has chosen to donate the funds to the trust, in appreciation of the crucial part grassroots venues had in his initial steps.
"As an artist wouldn't be doing my current career if it wasn't for all the shows I played around the region, and beyond, when I was just getting going," said the artist. "These locations are legendary, but they are struggling."
From early 2023 onward, over 150 of these spaces have permanently closed – roughly 16 percent of the entire UK sector.
Recently, well-known musicians including Pulp, Coldplay, Katy Perry and Sheeran have all supported the trust in its initiatives to support the industry, by adding a modest fee to their ticket costs, which benefits smaller concert halls.
Sam Fender also joined in, raising in excess of £100,000 on his recent tour to help numerous grassroots venues across Britain.
The donation supported locations that were confronting possible closure due to issues stemming from flooding, fire incidents and personal losses, as well as licensing issues, legal disputes and noise complaints.
Additional spaces obtained funding support in improving infrastructure and sound and lighting systems that positively impacted musicians and concertgoers.
"The concept that revenue from performances in big venues helps the smaller venues, where it begins for performers like me, is just sensible," Sam Fender has said.
The musician is among several artists to donate his Mercury Prize prize money to worthy causes.
Back when they earned the award in the mid-90s for their album the album, frontman the singer declared that the act would give their cash to the charity War Child.
During 2002, rapper and singer Ms Dynamite shared her award across multiple good causes, like the NSPCC and a charity for Sickle Cell.
"And I contributed a grand to Highgate Newtown, to their gymnastics class, because I practiced gymnastics when I was a child and they lacked updated gear," the artist explained to outlets.
Two years ago, they gave their winnings to the community center that supported their act, as well as other grassroots music organisations.
Also 1994 winners M People contributed their prize to a multiple sclerosis charity after a close contact was diagnosed the illness.
"The victory was quite enough," remarked singer Small. "The prize was the cherry on top but we didn't require the extra, because we had the cake. So our success touched somebody else's life."
Last week, the MVT revealed it had rescued a couple of grassroots venues in south-east England, by transferring them to community ownership.
The Joiners in Southampton and the second in the city were bought under the Own Our Venues scheme, which is funded by a national body and enthusiasts who acquire "shares" in the venues.
